Ngokuxhomekeke kuhlobo lwebhiya, usenokufuna ukuyisela ebhotileni kunetoti. Uphononongo olutsha lufumanisa ukuba i-amber ale iyahlaziyeka xa inxilwe ebhotileni ngelixa incasa ye-India Pale Ale (IPA) ayitshintshi xa idliwe ngaphandle kwetoti.

Ngaphaya kwamanzi kunye ne-ethanol, ibhiya inamawaka eekhompawundi zencasa ezenziwe kwiimetabolites ezenziwe ngamagwele, iihops, kunye nezinye izithako. Incasa yebhiya iqala ukutshintsha nje yakuba ipakishwe kwaye igcinwe. Iintshukumo zemichiza zophula iikhompawundi zencasa kwaye zenze ezinye, ezinegalelo ekwaluphaleni okanye incasa yebhiya endala efunyanwa ngabantu xa bevula isiselo.
Abenzi botywala kudala besebenza kwiindlela zokwandisa ubomi beshelufu kwaye baphephe ibhiya endala. Nangona kunjalo, uphando oluninzi malunga nokuguga kwebhiya lujolise kakhulu kwiilagi ezikhanyayo kunye neqela elincinci lemichiza. Kolu phononongo lwangoku, abaphandi kwiYunivesithi yaseColorado State bajonge ezinye iintlobo zebhiya ezifana ne-amber ale kunye ne-IPA. Baphinde bavavanya ukubona uzinzo lwekhemikhali yebhiya epakishwe kwiibhotile zeglasi ngokuchasene neenkonkxa ze-aluminiyam.

Inkonkxa kunye neebhotile ze-amber ale kunye ne-IPA zaye zafakwa kwisithuba senyanga kwaye zashiywa kwiqondo lobushushu begumbi ezinye iinyanga ezintlanu ukulinganisa iimeko zokugcina eziqhelekileyo. Rhoqo kwiiveki ezimbini, abaphandi bajonga i-metabolites kwizikhongozeli ezisanda kuvulwa. Njengoko ixesha lihamba, ukuxinwa kwee-metabolites-kuquka i-amino acids kunye ne-esters-kwi-amber ale yahluke kakhulu kuxhomekeke ekubeni ifakwe kwibhotile okanye i-can.

Uzinzo lweekhemikhali ze-IPAs aluzange lutshintshe xa lugcinwe kwitoti okanye kwibhotile, ukufunyaniswa ngababhali bacebisa ukuba kungenxa yokuxinana kwabo okuphezulu kwee-polyphenols ezivela kwi-hops. Iipolyphenols zinceda ukukhusela i-oxidation kwaye zibophelele kwi-amino acids, ezivumela ukuba zihlale kwibhiya kunokuba zibambeke ngaphakathi kwesikhongozeli.

Iprofayili ye-metabolic ye-amber ale kunye ne-IPA yatshintsha ngokuhamba kwexesha, kungakhathaliseki ukuba ifakwe kwibhokisi okanye ibhotile. Nangona kunjalo, i-amber ale kwiitoti yayinowona mahluko mkhulu kwiikhompawundi zencasa ngokuya yayigcinwa ixesha elide. Ngokwababhali bophononongo, xa izazinzulu zifumanisa ukuba i-metabolites kunye nezinye iikhompawundi ziyichaphazela njani iprofayili yencasa yebhiya, inokunceda ekwenzeni izigqibo ezinolwazi malunga nolona hlobo lulungileyo lokupakisha uhlobo oluthile lwebhiya.